Composite

Part:BBa_K3166058:Design

Designed by: Yijia Ren   Group: iGEM19_SEFLS_Shanghai   (2019-10-16)


IAY(rbs-idi-rbs-ispA-rbs-YSS)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 376
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1422
    Illegal SpeI site found at 376
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 376
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 376
    Illegal AgeI site found at 718
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR


Source

It is from E.coli BL21(DE3).


References